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实验室透析袋
℡ 4000-520-616
℡ 4000-520-616
echelon-inc/PKA cb, active/5ug/E-K033-5ug
产品编号:E-K033-5ug
市  场 价:¥3160.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$158.00
品      牌: Echelon
公      司:VISKASE
公司分类:
echelon-inc/PKA cb, active/5ug/E-K033-5ug
商品介绍

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CatalogNo.: E-K033

Description: Recombinantfull-lengthhumanPKAcbcontainingN-terminalGSTtagwasexpressedbybaculovirusinSf9insectcells.ThegeneaccessionnumberisNM_002731.
Thismaterialissoldforresearchpurposesonly.
SpecificActivity
29umolphosphateincorporatedintoCREBtidesubstrateperminutepermgproteinat30°Cfor15minutesusingafinalconcentrationof50µMATP(0.83µCi/assay).

ScientificBackground

  • MostoftheeffectsofcAMParemediatedthroughthephosphorylationoftargetproteinsonserineorthreonineresiduesbythecAMP-dependentproteinkinase(AMPK).TheinactiveholoenzymeofAMPKisatetramercomposedoftworegulatoryandtwocatalyticsubunits.ThemammaliancatalyticsubunithasbeenshowntoconsistofthreePKAgeneproducts:C-alpha,C-beta,andC-gamma.TwoPKAisoformsexist,designatedtypesIandII,whichdifferintheirdimericregulatorysubunits,designatedRIandRII,respectively.FurThermore,thereareatleastfourdifferentregulatorysubunits:RI-alpha,RI-beta,RII-alpha,andRII-beta.ThecAMPcausesthedissociationoftheinactiveholoenzymeintoadimerofregulatorysubunitsboundtofourcAMPandtwofreemonomericcatalyticsubunits.ThecatalyticsubunitC-betaofPKA(PKAcb)isamemberoftheSer/ThrproteinkinasefamilyandisacatalyticsubunitC-betaofAMPK.Berubeetal.assignedthePKAcbtohumanchromosome1bySouthernblotanalysisofsomaticc

    Storage

    • Storeproductfrozenatorbelow-70°C.Stablefor1yearat-70°Casundilutedstock.Aliquottoavoidrepeatedthawingandfreezing.

    References

    • 1.Berube,D.;Simard,J.;Sandberg,M.;Grzeschik,K.-H.;Gagne,R.;Hansson,V.;Jahnsen,T.:AssignmentofthegeneencodingthecatalyticsubunitC(beta)ofcAMP-dependentproteinkinasetothep36bandonchromosome1.(Abstract)Cytogenet.CellGenet.58:1850only,1991.2.Simard,J.;Berube,D.;Sandberg,M.;Grzeschik,K.-H.;Gagne,R.;Hansson,V.;Jahnsen,T.:AssignmentofthegeneencodingthecatalyticsubunitC-betaofcAMP-dependentproteinkinasetothep36bandonchromosome1.Hum.Genet.88:653-657,1992.
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    Viskase/透析袋 MD25(3500)/25mm/MD25-3.5MD25-3.5透析透析1543.14
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