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实验室透析袋
℡ 4000-520-616
℡ 4000-520-616
echelon-inc/PKC delta, active/5ug/E-K037-5ug
产品编号:E-K037-5ug
市  场 价:¥3160.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$158.00
品      牌: Echelon
公      司:VISKASE
公司分类:
echelon-inc/PKC delta, active/5ug/E-K037-5ug
商品介绍

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CatalogNo.: E-K037

Description: Recombinantfull-lengthhumanPKCdeltacontainingN-terminalGSTtagwasexpressedbybaculovirusinSf9insectcells.
ThegeneaccessionnumberisNM_006254.
Thismaterialissoldforresearchpurposesonly.
SpecificActivity
302nmolphosphateincorporatedintoCREBtidesubstrateperminutepermgproteinat30°Cfor15minutesusingafinalconcentrationof50µMATP(0.83µCi/assay).

ScientificBackground

  • ProteinkinaseCdelta(PKCdelta)isamemberoftheproteinkinaseC(PKC)familyofserine-threoninekinases.Itisa79kdproteinkinasethatshowsstrictdependenceonthepresenceofphospholipids,butshowsnoactivationbyCa2+(1).PhosphatidylinositolisthemostpotentactivatorofPKCdelta.Apparentkineticconstantsforsyntheticoligopeptides(MBP4-14,EGFRpeptideandepsilon-peptide)arequitedifferentbetweenPKCdeltaandotherPKCs.NorthernblotanalysisindicatedthatPKCdeltaiswidelydistributedinalmostallthetissuesandisamajorisoformofPKCexpressedinhemopoieticcells(2).PKCdeltaisinvolvedinfundamentalcellularfunctionsregulatedbydiacylglycerolsandmimickedbyphorbolesters.PKCdeltaispartiallyassociatedwiththeinsolublefractionincellsevenintheabsenceofphorbol12-myristate13-acetate(PMA).UponPMAstimulation,bothittranslocatetotheinsolublefractionofcellhomogenates(3).OverexpressionofPKC-deltainducessignificantchanges

    Storage

    • Storeproductfrozenatorbelow-70°C.Stablefor1yearat-70°Casundilutedstock.Aliquottoavoidrepeatedthawingandfreezing.

    References

    • 1.LeiberspergerH,GschwendtM,GernoldM,MarksF.Immunologicaldemonstrationofacalcium-unresponsiveproteinkinaseCofthedelta-typeindifferentspeciesandmurinetissues.Predominanceinepidermis.JBiolChem.1991Aug5;266(22):14778-84.2.MischakH,BodenteichA,KolchW,GoodnightJ,HoferF,MushinskiJF.MouseproteinkinaseC-delta,themajorisoformexpressedinmousehemopoieticcells:sequenceoftheCDNA,expressionpatterns,andcharacterizationoftheprotein.Biochemistry.1991Aug13;30(32):7925-31.3.MischakH,GoodnightJA,KolchW,Martiny-BaronG,SchaechtleC,KazanietzMG,BlumbergPM,PierceJH,MushinskiJF.OverexpressionofproteinkinaseC-deltaand-epsiloninNIH3T3cellsinducesoppositeeffectsongrowth,morphology,anchoragedependence,andtumOrigenicity.JBiolChem.1993Mar25;268(9):6090-6.
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    Viskase/透析袋 MD25(3500)/25mm/MD25-3.5MD25-3.5透析透析1543.14
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