ScientificBackground- ProteinkinaseCdelta(PKCdelta)isamemberoftheproteinkinaseC(PKC)familyofserine-threoninekinases.Itisa79kdproteinkinasethatshowsstrictdependenceonthepresenceofphospholipids,butshowsnoactivationbyCa2+(1).PhosphatidylinositolisthemostpotentactivatorofPKCdelta.Apparentkineticconstantsforsyntheticoligopeptides(MBP4-14,EGFRpeptideandepsilon-peptide)arequitedifferentbetweenPKCdeltaandotherPKCs.NorthernblotanalysisindicatedthatPKCdeltaiswidelydistributedinalmostallthetissuesandisamajorisoformofPKCexpressedinhemopoieticcells(2).PKCdeltaisinvolvedinfundamentalcellularfunctionsregulatedbydiacylglycerolsandmimickedbyphorbolesters.PKCdeltaispartiallyassociatedwiththeinsolublefractionincellsevenintheabsenceofphorbol12-myristate13-acetate(PMA).UponPMAstimulation,bothittranslocatetotheinsolublefractionofcellhomogenates(3).OverexpressionofPKC-deltainducessignificantchanges
Storage- Storeproductfrozenatorbelow-70°C.Stablefor1yearat-70°Casundilutedstock.Aliquottoavoidrepeatedthawingandfreezing.
References- 1.LeiberspergerH,GschwendtM,GernoldM,MarksF.Immunologicaldemonstrationofacalcium-unresponsiveproteinkinaseCofthedelta-typeindifferentspeciesandmurinetissues.Predominanceinepidermis.JBiolChem.1991Aug5;266(22):14778-84.2.MischakH,BodenteichA,KolchW,GoodnightJ,HoferF,MushinskiJF.MouseproteinkinaseC-delta,themajorisoformexpressedinmousehemopoieticcells:sequenceoftheCDNA,expressionpatterns,andcharacterizationoftheprotein.Biochemistry.1991Aug13;30(32):7925-31.3.MischakH,GoodnightJA,KolchW,Martiny-BaronG,SchaechtleC,KazanietzMG,BlumbergPM,PierceJH,MushinskiJF.OverexpressionofproteinkinaseC-deltaand-epsiloninNIH3T3cellsinducesoppositeeffectsongrowth,morphology,anchoragedependence,andtumOrigenicity.JBiolChem.1993Mar25;268(9):6090-6.
产品名 | 货号 | 公司分类 | 分类 | 美金价 | Viskase/透析袋 MD25(3500)/25mm/MD25-3.5 | MD25-3.5 | 透析 | 透析 | 1543.14 | 指尖陀螺 | 1221 | | 常用设备 | 4.50 |
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